The phospholipid bilayers are composed of phosphatidylcholine and cholesterol. All liposomal formulations are suspended in sterile phosphate buffered saline (PBS):

– 10 mM Na2HPO4;
– 10 mM NaH2PO4;
– 140 mM NaCl.

All our liposomes are slightly negatively charged and will therefore not clog together. The liposomes in the suspension are not sized, meaning that it contains smaller and larger liposomes as well (up to 3 micron). On average, the size of the liposomes are 1.7 micron.

Clodronate liposomes: a suspension of artificially prepared lipid vesicles encapsulating clodronate. The concentration of clodronate in the suspension is ca. 5 mg / mL. Clodronate is encapsulated in the liposomal vesicles in the form of CH2Na2Cl2O6P2 · 4 H2O.

PBS liposomes: a suspension of artificially prepared lipid vesicles encapsulating an aqueous PBS solution. These do not contain clodronate and can be used for control experiments.

Fluorescent DiI Liposomes: a suspension of artificially prepared lipid vesicles encapsulating an aqueous PBS solution, labelled with the fluorochrome DiI. These do not contain clodronate and can be used to investigate whether liposomes injected via a particular administration route are able to reach the macrophages to be studied.