LIPOSOMAL FORMULATIONS

CLODRONATE SUV PEG LIPOSOMES & CONTROL SUV PEG LIPOSOMES:

Clodronate SUV PEG Liposomes

Artificial spheres consisting of a single phospholipid bilayer encapsulating an aqueous PBS (phosphate buffered saline) solution containing clodronate. A special PEG-containing lipid is added to the composition, which allows for long circulation in blood and slower degradation of the formulation. The Liposomal suspension has been extruded and sized to 200nm in diameter and will not tend to precipitate upon storage. The concentration of clodronate in the suspension is ca. 5 mg/ml.

Upon request, the size can be reduced to 120-150nm with an extra extrusion step.

Control SUV PEG Liposomes

Control liposomes do not contain clodronate. Containing PBS only, they can be used for control experiments, in order to find out whether the effects observed after injection of clodronate liposomes are due to macrophage depletion exclusively. The Liposomal dispersion has been prepared with a special PEG-containing lipid which allows for long circulation in blood and slower degradation of the formulation. This formulation has been extruded and sized to 200nm in diameter which makes it the preferred control for the Clodronate SUV PEG liposomes.

Upon request, the size can be reduced to 120-150nm with an extra extrusion step.