ZenBio human pre-adipocytes (adipose derived adult stem cells) and placental mesenchymal stem cells (MSC) are characterized by their self-renewing capacity and ability to differentiate into chondrocytes, adipocytes, and osteocytes. This makes them attractive starting materials for tissue engineering and regenerative medicine applications.
Since few transplanted cells persist in vivo, the beneficial effects of cell therapy may lie in the secreted factors being the active component of this treatment. A key part of paracrine secretion is Exosomes, which are membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane.
Quality Control: All cells have been screened negative for HIV-1, HIV-2, Hep-B, Hep-C and Syphilis. All isolated and concentrated exosomes undergo includes Particle mean diameter, Protein concentration, RNA concentration and Concentration of particles/ml.
After the cell monolayer was 'wounded' using a cell scraper, the cultures were washed and fed with DMEM + 10% exosome-free FBS (Control), or DMEM + 10% exosome-free FBS supplemented with 25µg of pre-adipocyte exosomes (Exosome Treated). After a 5 day incubation, fibroblast proliferation closed the gap between the two edges of the wounded monolayer only in the medium supplemented with exosomes. These data support the notion that factors including exosomes secreted by pre-adipocytes (adipose tissue-derived MSC) play a role in wound healing and tissue regeneration, by facilitating cellular proliferation
![]() |
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |