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Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |
Renal Disorders
Life Technologies offers cell-based nephrotoxicity assays to test and identify compounds with potential renal toxicity. The kidneys play a crucial role in eliminating drug metabolites, and drug-induced nephrotoxicity can have severe consequences. With a team of highly experienced experts in the field of drug discovery, Life Technologies is equipped to assist you throughout the entire process. We can help with study design, experiment conduct, and data analysis to evaluate the nephrotoxicity of your compounds at an early stage of drug discovery. Screening drug candidates for nephrotoxicity is of utmost importance to rule out renal toxic drugs and prevent adverse incidents during pre-clinical and clinical stages.
At Life Technologies, we are committed to supporting the development of safer and more effective medications. Our renal toxicity assays using HRPTEpiC or HREpC offer valuable insights to help you make informed decisions and reduce the likelihood of nephrotoxicity-related drug failures.
Cytotoxicity Assay: Life Technologies utilizes HRPTEpiC cells in the in vitro assay to evaluate compound-induced nephrotoxicity. Cell viability, apoptosis (caspase 3), and mitotic block (phosphorylated Histone H3) markers are assessed using high-content analysis for mechanistic insights.
Mitochondrial Stress Assessment: Life Technologies employs high-content imaging to simultaneously detect and monitor indicators of mitochondrial stress and cell death, such as mitochondrial membrane potential, Cytochrome C release, caspase 3, c-Jun, and morphological parameters.
Biomarker (KIM-1 and NGAL) Detection:Life Technologies utilizes kidney injury molecule-1 (KIM-1) as a sensitive and renal-specific biomarker for early detection of renal proximal tubule injury. We quantify KIM-1 expression levels to predict nephrotoxicity. Additionally, we assess the overexpression and release of neutrophil gelatinase-associated lipocalin (NGAL) in cultured kidney cells as a biomarker of renal toxicity.
Life Technologies is capable of conducting multiplexed nephrotoxicity assays using human renal epithelial cells. Our team of experts can design customized assays to meet your specific requirements. Contact us to learn more about our capabilities in drug toxicity screening.
Discovery Services:
- Cytotoxicity Assays
- Life Technologies includes measurement of cell health markers like GSH, PLD, mito mass, MMP, and ATP levels.
- Mitochondrial Stress Assessment
- Biomarker (KIM-1 and NGAL) Detection
- High Content Screening- Using standard cell model and disease-related cell lines.
- High Content Imaging- Analysis of drug interaction at a sub-cellular level
Available Models:
- Renal Proximal Tubule Epithelial Cells (RPTEC)
- 3D models of Human iPSCs
- Human Primary cell-based 2D and 3D disease-relevant models
Techniques Used: Flow Cytometry, Confocal/Fluorescence Microscopy (Multi-colour Immunofluorescence), ELISA (Fluorimetry/Colorimetry), RTqPCR, WB.