Efficiency and safety assessment play a crucial role in the process of drug discovery. Before progressing to animal testing, it is important to utilize reliable and cost-effective in vitro assays that can serve as indicators of the absorption, distribution, metabolism, and excretion (ADME) fate of compounds in vivo. At our organization, we specialize in providing a comprehensive and adaptable range of in vitro DMPK/ADME studies. These studies are specifically designed to cover a wide range of activities from the hit-to-lead and lead optimization phase all the way to candidate selection.
At Life Technologies, we offer a range of in vitro assay formats that cater to the diverse needs of drug discovery and development. Our flexible ADME screening approach is designed to accommodate different stages of the pipeline, allowing for the characterization of chemical series and the determination of metabolism and drug interactions for compound candidates.
At Life Technologies, we deeply value your needs and prioritize delivering exceptional customer care. We understand the importance of providing quick and attentive support to ensure your satisfaction throughout our collaboration.
At Life Technologies, we offer compound profiling services aimed at improving the physicochemical characteristics of your compounds. Our comprehensive analysis includes solubility assessment, plasma protein binding, permeability, absorption, stability, and reactivity studies for drugs.
Log D and Log P assessments help in understanding the distribution and partitioning behavior of compounds, providing insights into their potential uptake and distribution within the body. Kinetic and thermodynamic solubility studies evaluate the solubility properties of compounds under different conditions, providing critical information for formulation and dosage form optimization.
In Vitro Metabolism:
At Life Technologies, we specialize in assessing the efficacy and safety of drugs through metabolic assays. Our metabolic profiling services support the calculation of drug efficacy, dosage rates, and safety profiles. We employ a range of advanced techniques and utilize liver microsomes, S9 fractions, and hepatocytes to obtain precise and accurate data on in vivo clearance mechanisms.
We specialize in determining the involvement of specific cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGTs) in the metabolism of drugs through reaction phenotyping. This information helps understand the metabolic pathways and potential drug-drug interactions.
At Life Technologies, we understand that the study of drug-metabolizing enzyme inhibition and induction is crucial in assessing metabolic drug-drug interactions. When multiple drugs are administered concomitantly, they can lead to altered drug exposures, either increasing or decreasing plasma levels. Inhibition or induction of cytochrome P450 enzymes can significantly impact the plasma levels of administered drugs, thereby influencing their efficacy or toxicity.
At Life Technologies, we offer a wide range of services to investigate and characterize drug metabolism and drug-drug interactions. Our expertise includes cytochrome P450 (CYP) and UGT reaction phenotyping, cytochrome P450 induction, cytochrome P450 inhibition, cytochrome P450 relative induction score, cytochrome P450 Ki determination, activation of nuclear receptors such as PXR and AhR, time-dependent inhibition (TDI) studies, reaction phenotyping for CYP identification, and UGT inhibition studies.
At Life Technologies, we specialize in conducting plasma protein binding assays, which play a crucial role in understanding drug distribution and determining the unbound fraction of drugs in plasma proteins. These assays provide valuable data that, when used in conjunction with pharmacokinetic and pharmacodynamic modeling, aid our clients in predicting drug-drug interactions more accurately.
Samples we study with:
Life Technologies provides drug distribution assays in plasma, tissue homogenate, blood, furin microsomes, and furin hepatocytes.
Permeability and Transporters:
At Life Technologies, we offer a comprehensive range of assays to measure the oral absorption properties of drugs, including permeability, transport across cell membranes, and bioavailability. Our services are highly customizable, ensuring that we meet your specific requirements and deliver reliable results.
Life Technologies provides assays for drug permeability and transport, including GIT-PAMPA, BBB-PAMPA, Caco-2, BCRP-MDCK-II, MDR1-MDCK-II, and LLC-PK1-MDR1. Reliable results for drug research.
Cytochrome P450 Induction:
Cytochrome P450 (CYP) induction assays assess test compound metabolism and estimate potential clearance pathways through reaction phenotyping methods.
HepG2 cell line (CYP3A4)-reporter gene assay and human primary hepatocyte-based assays evaluate drug metabolism.
Metabolite Identification and Biotransformation:
We identify and analyze metabolites to assess drug safety, performing comprehensive structural data analysis using advanced instrumentation.
We offer intrinsic clearance (CLint) and extensive profiling of top three metabolites in liver microsomes and hepatocytes, along with metabolite soft spot analysis, fingerprinting, and high-throughput reactive metabolite screening.
We utilize liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) and mass spectrometry (MS).
In Vivo Pharmacokinetics:
We provide in vivo pharmacokinetics (PK) services to study the ADME of drugs, supporting drug discovery, selection, efficacy, and development. Our assessments include plasma exposure, bioavailability, and dosing frequency, shedding light on drug efficacy. We offer a wide range of drug administration routes and use mice, rats, and rabbits as animal models. Our PK/PD services boast excellent turnaround times and competitive pricing.
We specialize in conducting bioequivalence PK studies in rodents, cassette dosing pharmacokinetics, mass balance pharmacokinetics, tissue distribution studies, dose proportionality pharmacokinetics, mechanistic PK models, in vivo drug-drug interaction (DDI) studies in rodents, serial blood sampling in mice, intravenous infusion in jugular vein cannulated mice and rats, and crossover pharmacokinetics in dual vein cannulated rats
We offer rapid formulation preparation for single-dose intravenous pharmacokinetic studies, providing solutions, suspensions, creams, emulsions, ointments, gels, and more.
We specialize in advanced bioanalytical method development and qualification, including automated sample handling. Our expertise covers sensitive small molecules, light-sensitive, and cytotoxic compounds, conducting bioanalysis in multiple bio-matrices for tissue distribution studies.
Dried blood spot (DBS) analysis, bioanalysis of stereochemical compounds, pharmacodynamic (PD) biomarker assessments, and biomarker modifications in intricate pre-clinical and clinical biological substrates, including plasma, serum, urine, tissue extracts, and cerebrospinal fluids, are all critical areas in modern biotechnology and pharmaceutical research.
PK/PD Modelling and Simulations:
Pharmacokinetic/pharmacodynamic (PK/PD) modeling and simulation techniques are invaluable in determining suitable dosage regimens for efficacy studies. These mathematical models offer a reliable and exact estimation of drug behavior in the body.
Understanding the relationship between PK, PD, and efficacy, whether it's based on overall drug exposure or peak plasma concentration, is crucial in determining the optimal dose and dosing regimen of a new drug. This involves a detailed study of the drug's absorption, distribution, metabolism, and excretion (ADME) properties.
Large Molecule Analysis:
Our laboratory employs state-of-the-art mass spectrometry techniques for the analysis of macro molecules. Our assays incorporate two main strategies, namely the top-down method involving comprehensive analysis of intact molecules, and the bottom-up method which relies on enzymatic digestion, primarily using trypsin, before the examination of the resultant peptides.
For tailored in vitro ADME/DMPK services, you're encouraged to reach out to us at firstname.lastname@example.org. Our team at Life Technologies is equipped to support and guide you in fulfilling your unique requirements.