 |
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |
Neuronal Disorders
The most fascinating organ in the body has always been the brain. Despite considerable developments in neuroscience, the diseases and ailments associated with the brain have long been a mystery. Finding treatments for crippling conditions that damage the body's central nervous system requires collaboration. The Life Technologies team is well-equipped with the top research scientists and infrastructure. We provide our clients with a range of services to assist them create treatments and give their research program significant weight.
BBB Model: Monitor Cell-Cell Interactions Between Vascular and Brain Cells
Discovery Services:
- Real-time drug permeability across the BBB.
- Neurotoxicity on the cells of the BBB.
- Evaluate drugs that have a protective effect on the BBB
- Investigate the effects of tumor cells on the BBB
- Evaluate inflammatory effects on the BBB
- Biomarker Analysis
- Visualize and quantify the migration of immune cells
- Genomic, proteomic, and metabolic analysis of normal and dysfunctional BBB.
Models Available:
- Monoculture with brain endothelial cells
- Co-Culture with brain endothelial cells and astrocytes
- Tri-Culture with brain endothelial cells, astrocytes, and pericytes
Neurodegenerative Diseases: Alzheimer's and Parkinson's Disease
The main features of neurodegenerative illnesses, which are extremely crippling ailments, are gradual neuronal death and nervous system damage. The most prevalent neurodegenerative disorders are Alzheimer's and Parkinson's, and as global average life expectancy rises, so does the severity of these conditions. Drug research projects benefit from cell models that reflect the characteristics of the disease because they reveal information about the therapeutic advantages of developing medications. At Life Technologies, we offer in vitro cell models to research the genetic and molecular causes of Parkinson's and Alzheimer's disease with the goal of identifying disease-modifying therapies to treat neurodegenerative disorders. Additionally, we recommend phenotypic screening as a crucial method for finding new medicinal compounds. From straightforward monoculture cell models to high-throughput three-dimensional cell models, we provide a variety of cell-based assays for drug development.
Discovery Services
- Tau levels/ clearance assays
- Toxic Aβ1-42 peptide exposure
- LDH release measurement
- NeuN cell viability assay
- Alpha-synuclein aggregation quantification
- Neuronal mitophagy assays (Tom20 Loss Quantification)
- Cytoprotective assays
Available In-Vitro Model
- Rodent primary mixed cortical neurons
- Rodent primary hippocampal neurons
- Rodent primary midbrain-TH-enriched neurons
- Human potassium ion channel expressing cell lines
- Human M1 Muscarinic Acetylcholine Receptor (GPCR) Cell Lines
- Human NMDA receptor Cell lines
- Human post-mortem microglia cultures
- hiPSC-derived cortical neurons
- Human M5 Muscarinic Acetylcholine Receptor (GPCR) Cell Lines
- Human ReNcell VM and SH-SY5Y cell lines with dopaminergic features
- Rodent and human astroglial & microglial cell cultures
- Human iPSCs derived brain cells
- Human NSCs
- 3D Organoids of neuronal cell lines
Neuro-Inflammation:
In numerous neurodegenerative illnesses, including Alzheimer's disease, Parkinson's disease, stroke, multiple sclerosis, etc., neuroinflammation is now understood to be a crucial component. Due to the large number of proinflammatory mediators that astrocytes and microglia release, including nitric oxide, cytokines, and chemokines, they are important participants in neuroinflammation. The Life Technologies team's range of tasks in in vitro cell-based models and assays includes target validation, sophisticated cellular screening, mechanism of action studies, and early translational biology research.
Discovery Services:
- Lipopolysaccharide (LPS)-induced inflammation
- Nitric oxide production
- Microglia activation
- Macrophage activation
- Phagocytosis Assays
- Human microglia assays: cytokine release & calcium imaging
- Neuronal and microglial inflammation assays
- Microglial activation & phagocytosis studies
- Neuro-Glia co-cultures studies
- Cytokine release assays
- LPS-induced inflammation
- Neurite outgrowth assays
Available Models:
- Adult murine microglia
- iPSC-derived astrocytes/ neurons
- Murine cortical glial and mixed cultures
- Human microglial cultures
- Human Potassium & Sodium ion channel expressing cell lines
Techniques Used: Flow Cytometry, Confocal/Fluorescence Microscopy (Multi-colour Immunofluorescence), ELISA (Fluorimetry/Colorimetry), RTqPCR, WB.