Life Technologies

Cancer, immunological disorders, and other infectious diseases are among the problems that large molecules are utilized to help cure. Our platform includes all early-stage big molecule discovery studies and takes it all the way to drug development.

To fulfill your demands for drug discovery, Life Technologies provides a wide range of integrated discovery biology services. Recombinant DNA technologies, protein sciences, cell-based assays, DMPK, toxicology, and other in vitro assays are some of the services we offer.

Recombinant DNA Technology:

In the areas of recombinant DNA technology, antibody gene sequencing, mutagenesis, library production, plasmid DNA preparation, microbial genome engineering, gene expression analysis, and IVT mRNA synthesis, we support and provide a wide range of services.

Cloning and Vector Generation:

Ligation dependent and independent cloning
Recombination based high throughput cloning
De novo cloning (Synthetic gene fragments, PCR and cDNA based)
Customized vector construction based on client requirement

Plasmid DNA Preparation:

Small research institutions as well as large-scale manufacturing for biotech and pharmaceutical corporations are both served by the plasmid DNA purification service;

Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production.
High quality industrial grade plasmid suitable for protein, antibody production, stable cell line generation in mammalian cells, bacmid generation for protein expression in insect cells, viral packaging, in vitro mRNA synthesis, vaccine, gene therapy studies and animal immunization.
Process development for customized plasmid propagation and purification.

Mutagenesis:

Customized mutagenesis specifications, including the creation of mutant libraries and site-directed and random mutagenesis.
Point mutations, insertions, deletions, or substitutions of gene fragments.
Alanine scanning, combinatorial mutagenesis, site saturation mutagenesis, and gene shuffle.

Antibody Gene Sequencing and Reformatting:

Life Technologies offers a one-stop shop for the recombinant expression and creation of antibodies through the sequencing and reformatting of antibody genes.

RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation.
Degenerate oligos.
De novo antibody sequencing and analysis using LC-MS/MS.
Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, VHH/Nanobody.
Antibody gene sequencing for Mouse, Rat, Rabbit and other species.

Strain Engineering:

BSL-1 and BSL-2 microbes

Gene knockout and knockin techniques based on homologous recombination.

Characterization of engineered strains using microbiological methods, RT-PCR and advanced analytics.

CRISPR-Cas9 based mutagenesis.

Construction of cDNA, phage, and yeast libraries, as well as plans for high-throughput screening and functional characterization, constitute library generation and evaluation.

Gene Expression Analysis:

Life Technologies' researchers use a variety of techniques to conduct regular expression investigations;

Quantitative real time PCR.
ELISA in various formats.
Flow Cytometry.
Western blotting.
Mass Spectrometry.

Residual Protein, DNA and Mycoplasma Detection:

In a recombinant biotherapeutic, residual host-cell proteins (HCP) and host-cell DNA (HCD) are contaminants. We make sure that these pollutants and impurities are found and reduced to levels below those recommended by regulatory organizations.

Quantitative Real time PCR
ELISA
SPR (Biacore)
Mycoplasma test

Protein Sciences

We provide a wide range of services, from sequence optimization to creation of the finished product (Gene to Protein). In order to better serve our clients, we provide the following services and solutions with a variety of flexible access points.

Our Protein Sciences Services Include:

Protein Expression:

We offer

Designing, scouting and screening studies for challenging proteins.
Protein expression studies.
With low running expenses, scale up using the spinner flask, shaker flask, HYPER flask, and WAVE bioreactor platforms to handle volumes up to 50 L scale.

Mammalian Expression Systems:

Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc.
Protein production in transient expression mode or using stable clones or pools.

Insect Cell Expression Systems:

Insect cell lines from the Sf9, Sf21, Hi5, and super Sf9 families were used to express and produce proteins via baculoviruses.

Yeast Expression Systems:

Recombinant molecules are normally released in medium by genome integration, resulting in stable protein expression in Pichia.

Bacterial Expression Systems:

Soluble, periplasmic and inclusion bodies in E. coli
Multi-protein expression by co-transfection or duet plasmid technology
Refolding condition screening and method optimization

Protein Purification:

We are skilled at purifying all classes of target proteins from mammalian, yeast, and microbial expression systems, both tagged and untagged.

Purification process optimization
Automated FPLC systems and batch binding protein purification
IMAC, IEC, mixed-mode, HIC, SEC, and affinity for micrograms to gram-scale quantities are chromatographic purification techniques.

Antibody Purification:

Purification of monoclonal antibodies from various sources, including human and non-human primates, mice, rats, pigs, gerbils, and more.
Generation from full-length mAbs and purification of various antibody fragments (Fab, Fab2, F(ab)'2, VHH, scfv, etc.)
Bi-specific and KIH antibodies
Diabodies
Single domain antibody
Protein purification from hybridoma cultures

Microbial Purification Capabilities:

Soluble protein
Periplasmic protein
Intracellular protein
Refolding from inclusion bodies
Purification of Major Histocompatibility (MHC) complexes
Membrane protein production - GPCRs, nanodisc technology, etc.
Antibody drug conjugate screening, development and purification
High throughput protein screening and purification
Protein cleavage and purification

Protein Analytics and Characterization

Throughout all project stages, we regularly analyze proteins to ensure their authenticity, purity, structural and conformational integrity, stability, and functionality as part of our commitment to delivering high-quality goods.

Quantitative analysis: Protein estimation using Absorbance at 280nm,Bradford and BCA assays
Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blotanalysis
Purity: SE-HPLC, IEX-HPLC and RP-HPLC
Protein stability analysis (Real-time and Accelerated Stability Testing)
LC-MS analysis
Capillary electrophoresis (CE).
Peptide Mass Fingerprinting (PMF)
Biological activity and binding studies using enzymatic assays, ELISA, Biacore, etc.
Endotoxin analysis
Host cell protein (HCP) and Host cell DNA (HCD) analysis

Protein Stability Analysis

Real-time and accelerated stability testing.
Long-term stability studies.
Purity: SE-HPLC, IEX-HPLC and RP-HPLC

Protein Conjugation and Labelling

Protein labelling/conjugation (HRP, biotin, Ruthenium, fluorescent labels, etc.)
Conjugation of resin with specific protein/peptide

SERVICES

Large Molecules Discovery Services