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Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |
| Coveted Services | ||||||
| Service Name | Description | Deliverable | ||||
|---|---|---|---|---|---|---|
| Hybridoma Cell Line Development | Development of Murine Hybridoma Cell Lines against an Antigen. Delivery of 5 Positive Cell Lines. | 5 Hybridoma Cell Lines | ||||
| Antibody Production: 1) Monoclonal Antibody Production in Ascites (Mice) 2) Monoclonal Antibody Production (Hybridoma Cell Culture) - Proprietary Method to Increase Yield 3) Recombinant Antibody Production in CHO Cells from Sequence 4) Polyclonal Antibody Production (Rabbit or Goat) |
Variety of Antibody Production Methods for Delivery of Purified Monoclonal or Polyclonal Antibodies | Purified Antibody | ||||
| Development of Recombinant Producing Protein Cell Lines in Yeast or E.Coli | Gene Synthesis, Transformation, and Culturing/Stabilization of Recombinant Producing Protein Cell Lines in Yeast or E.Coli. Functional Validation Available. | Cell Line and SOP via Tech Transfer | ||||
| GMP Manufacturing of Recombinant Proteins | Fermentation/Cell Culture Based Production of Recombinant Proteins; Available in ISO 13485/GMP Manufacturing. | Purified Protein | ||||
| 1) Protein A Purification of Antibodies 2) Protein G Purification of Antibodies 3) Immunoaffinity Column Development and Purification of Antibodies 4) Affinity Chromatography Purification HPLC, for Proteins | A Range of Affinity Chromatography-Based Purification Options | Purified Antibody in Buffer with QC Report | ||||
| 1) HRP Conjugation 2) ALP Conjugation 3) Biotin Labeling |
Labeling of Antibodies with various Enzymes for use in Diagnostic Applications | Conjugate with QC Report | ||||
| Epitope Binning: Develop Matched Pair of Antibodies for Use in Assays | Screening for Matched Pairs from Min. Pool of 5 Antibodies. | Matched Pair of Monoclonal Antibodies | ||||
| Total Development and Validation of ELISA Kit. | Total Development of ISO 13485 Compliant ELISA Kit against any target antigen. Multiplex ELISA development also available for 2 Antigens. | Functionally Validated ELISA Kit + SOP | ||||
| Total Development and Validation of Rapid Lateral Flow Strip. | Total Development of ISO 13485 Compliant LFS Kit against any target antigen. Multiplex development also available up to 3 Antigens. | Functionally Validated LFS Kit + SOP | ||||
| Exosome related Services | Exosome isolation from cell culture medium Exosome isolation from serum Dynamic Light scattering for exosome or nanoparticle size and zeta potential detection) (DLS analysis) Nanoparticle Tracking analysis to detect number of exosome particle & size in 1 ml sample) Transmission electron microscopy to detect structure and size of exosomes or nanoparticle HPLC assay to quantify amount of drug loading in exosomes/ nanoparticles Drug or natural compound packaging in exosomes (exogeneous packaging) | 109 exosomes sterile 109 exosomes sterile DLS analysis report for samples Nanoparticle Tracking analysis report for samples Test report Test report per compound in 107 exosomes + HPLC analysis | ||||
| MTT Assay Service | Testing the toxicity of new anti-cancer molecules on primary tumor cells, CTCs (MTT Assay) Testing the toxicity of new anti-cancer molecules on Cancer stem cell (3D culture) derived from cell lines (MTT Assay) MTT assay (cell cytotoxicity assay) in a single cell line with 1 test compound) Cancer stem cell characterization using CSC specific markers from a single cell line Confocal microscopy to detect cellular apoptosis using AnnexinV Generation of mature dendritic cells from monocytes PBMC from Human blood 15 million cells (Healthy donor) Flow cytometry analysis to detect Ki67 and AnnexinV ELISA assay |
Test report of molecule in triplicate Test report of molecule in triplicate Test report of molecule in triplicate For 2-3 CSC specific markers using FACS analysis Test report 10⁶ cells 1 vial of PBMC with 15 million cells 1 sample per antibody Test report | ||||
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Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |