BNCC Cell Lines
BNCC Cell Lines & Related Products
Precision. Traceability. Reliability.
Accelerating cell-based research with authenticated cell lines, cryopreserved cells, complete culture media, fetal bovine serum, and quality documentation from BNCC.
Why Choose BNCC?
Cryopreserved Cells
Mycoplasma Testing & COA
Research Applications
APPLICATIONS
STR Testing Report
1. Sample Information
2. Test Method
Test method: Extract DNA using the Tian Gen genome extraction kit, amplify using the 21-STR amplification protocol. Detect STR loci and the sex gene Amelogenin on the Seqstudio genetic analyzer.
3. Test Results
1) Basic Test Results
| Multi-allele | Matched Cell Line | Cell Bank | EV Value | Match Description |
|---|---|---|---|---|
| None | MRC-5 | ExPasy | 1.00 | Perfect Match |
Multi-allele refers to the phenomenon of three or more alleles. The typing results of each cell inthis test were good.
2) Sample description:
The DNA typing of this cell line was matched perfectly in the cell line search, and the ExPASy database shows that the cell name is MRC-5 and the cell number corresponds to CVCL-0440. No multiple alleles were found in this cell line during this test.
3) Matching description
Compare the STR data of the tested cell line with the cell lines included in ATCC, DSMZ, JCRB, and ExPASy databases. Cell lines not included in the above databases will not be matched.
MYCOPLASMA TEST REPORT
qPCR Based Detection
| Channel | CT Value |
|---|---|
| FAM | N/A |
| ROX | 28.11 |
BASIS
| FAM Channel | ROX Channel | Interpretation |
|---|---|---|
| Positive control | Ct<35 | Reagents works |
| Positive control | Ct>35 | Reagents not work |
| Sample no Ct | Ct<35 | Negative |
| Sample Ct<40 | Ct<35 | Positive |
| Sample no Ct | Ct>35 | PCR inhibition |
CERTIFICATE OF ANALYSIS
CELL INFORMATION
INSTRUCTIONS
If there is any quality problem to the products, please contact the customer service within 24 hours, or it will be considered as normal for the products. For the Frozen cell, it shall be stored in the refrigerator at -80°C upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction. Otherwise, the replacement of cells are not be available in case of loss of cell viability.
MEDIUM
- DMEM-H complete medium (containing 10% FBS);
- DMEM-H complete medium: 90% DMEM-H + 10% FBS
Viability
Adherence is observed in 18 hours, the cell adherence rate ≥ 80.0% in 80
Morphology
Epithelial cell like, short spindle-shaped, polygonal, irregular edges, monolayer adherent growth
Recovery record
According to the recovery instructions, the results of the cell recovery are reported as follows:
RECOVERY STEPS
The frozen vial is taken out of liquid nitrogen or -80 °C refrigerator and put into PE gloves, quickly submersed into a 37 °C water bath, shaken the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min.
Put the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 5min, discard the supernatant, resuspend the cells with 1-2ml of complete media.
The cell suspension was added to T25 flask containing 5-6ml complete medium and cultured in an incubator.
SUBCULTURE / CRYOPRESERVATION
remove the medium, rinse twice with PBS, and add 1-2mL pancreatin (0.25% Trypsin + 0.02% EDTA);
Observe the digestion situation under the microscope. When the cell edge shrinks and the adherent is loose (a pasteur pipette can be used to suck up some pancreatin and gently blow somewhere in the cell layer, and the cell layer can be seen to detach with naked eyes, I .e. digestion is completed, otherwise digestion is continued), directly suck out pancreatin, add 5-6mL of complete medium, gently blow the cell layer off.
dispense the cell suspension into a fresh T25 flask as the ratio of 1:2, add appropriate complete culture medium, make the cell suspension well distributed, and culture in an incubator.
Pay attention to the change of pH value of media and cell density, renew the media regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80%-90%.
MICROSCOPY IMAGES
NOTES
The cells are density dependent. Initiate the subcultivation in T25 flask when the cell density reaches 80%; A subcultivation ratio of 1:2 is recommended.
If the cells in sealed culture bottle, put it into the incubator for cultivation after handling, with the cap unscrewed.
