The EnzSAP™ Reagent is comprised of two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I). The combination of these enzymes ensures complete dephosphorylation of unincorporated dNTPs and degradation of residual primers, enabling Sanger Sequencing and other downstream applications. The reagents are active in commonly used PCR buffers, which eliminates the need for buffer exchange.