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Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |
DESCRIPTION
Human Pulmonary Artery Endothelial Cells (HPAEC) provide an excellent model system to study many aspects of cardiovascular function and disease, including diabetes-associated complications, mechanisms of endothelial dysfunction caused by environment, oxidative stress, hypoxia and inflammation, as well as mode of action of various natural compounds and drug candidates.
Note that several publications describe the differences between endothelial cells from different vascular beds and between macro- and micro-vascular cells from the same organ, highlighting the importance of confirming any new findings on cell lots obtained from several different origins. Life Technologies (India) Pvt Ltd., Inc. offers the widest variety of endothelial cells, and is well equipped to meet this need.
HPAEC from Life Technologies (India) Pvt Ltd. have been used to investigate or demonstrate
- Vasculoprotective effects of propofol, demonstrating its potential for treating pulmonary arterial hypertension
- Hits from a high-throughput screening assay and discover anziaic acid inhibits bacterial activity
- Endothelin-1 is released by endothelial cells during acute crisis in sickle cell anemia, increases vascular constriction
- Pollutant particles cause vascular dysfunction by up-regulating clotting-related genes
- Central role of AMP-activated protein kinase in normal endothelial barrier function can be disrupted, causing endothelial hyperpermeability and lung injury
- Anti-inflammatory and cardioprotective effects of resveratrol
- cGMP prevents oxidant-induced damage to the endothelial barrier function
- Radiation causes endothelial cell senescence due to up-regulation of proliferative signaling in the presence of cell cycle arrest
- Vanadium exposure causes pulmonary vasoconstriction mediated in part by the inhibition of endothelial NO production
- Cytotoxic effects of a novel pore-forming protein, proposed as an anti-tumor agent
- Effects of Bone Morphogenic Protein-4, helping to explain why its upregulation leads to atherosclerosis and hypertension
- Macro-vascular pulmonary endothelial cells accumulate HIF-1 under hypoxic conditions
- Transportation of bioactive lipids and its silencing by siRNA
(Click to Enlarge) Human Pulmonary Artery Endothelial Cells: HPAEC from bone marrow (A) differentiating into multiple lineages. Adipogenic - Oil Red O staining (B), Chondrogenic - Alcian Blue Staining (C) and Osteogenic - Alzarin Red S staining (D).
DETAILS
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Tissue
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Normal healthy human pulmonary artery
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Character
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VIII-related Ag expression, DiI-Ac-LDL uptake
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 HPAEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HPAEC (302-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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At least 15
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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INSTRUCTIONS HPAEC
Format: PDF
PRODUCTS
| PRODUCT | SIZE | CAT.# |
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| Cryopreserved Human Pulmonary Artery Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 302K-05a |
| Cryopreserved Pulmonary Artery Endothelial Cells (HPAEC), adult: Frozen HPAEC (5x10^5) | 1 Cryovial | 302-05a |
| Cryopreserved Pulmonary Artery Endothelial Cells, Asthma (HPAEC-AS), Pre-Screened, adult: Frozen HPAEC-AS from donor with Asthma (5x10^5) | 1 Cryovial | S302AS-05a |
| Cryopreserved Pre-Screened Human Pulmonary Artery Endothelial Cells with Asthma Total Kit: 5x10^5 Cells (from donor with Asthma, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S302ASK-05a |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | T-25 Flask | 303-25a |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | T-75 Flask | 303-75a |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | 24 Well | 303-24Wa |
| Proliferating Pulmonary Artery Endothelial Cells (HPAEC), adult: Actively growing, dividing cells in medium | 96 Well | 303-96Wa |
RESOURCES/DOCUMENTS
5 IMPORTANT CELL CULTURE RULES
Format: PDF