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Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400 Email# customerservice@lifetechindia.com |
DESCRIPTION
Human Bronchial Epithelial Cells (HBEpC) cprovide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. When grown on inserts and provided with the liquid/air interface, HBEpC can differentiate into a pseudostriated epithelium and serve as a more physiological 3D tissue model for in vitro studies. The HBEpC shown here were cultured (L) and immunolabeled for cytokeratin 18 (R).
Below, HBEpC from Life Technologies (India) Pvt Ltd. are stained with ACE2 and KRT5. Ongoing research suggests ACE2 is the cellular receptor utilized by SARS-CoV-2, the coronavirus that causes COVID-19 , for cell entry.
(Click to Enlarge) Human Mesenchymal Stem Cells (HMSC) from bone marrow (A) differentiating into multiple lineages. Adipogenic - Oil Red O staining (B), Chondrogenic - Alcian Blue Staining (C) and Osteogenic - Alzarin Red S staining (D).
HBEpC from Life Technologies (India) Pvt Ltd., Inc. have been used to examine:
- Activation, expression and production of genes, kinases and signaling pathways by cytokines, growth factors, interleukins, binding proteins and pro-inflammatory molecules.
- Stimulation-dependent, observable changes in proliferation, bronchial epithelial permeability, crosslinking of membrane glycoproteins and cell surface adhesion molecules. Drug discovery cell screening for in vitro assay of compounds, or to extend and confirm high-throughput work done in cell lines.
- Clinical focused discoveries leveraging HBEpC include therapeutics to suppress tumor gene transcription, apoptosis, inflammation, auto-immune disease and viral infection, while enhancing cell protection, repair and lifespan.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
DETAILS
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Tissue
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Human bronchial epithelium: nondiseased, Asthma, COPD, or Type 2 Diabetes
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 HBEpC (1st passage) frozen in Basal Med w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HBEpC (502-05a), Gr Med (511-500), Subculture Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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At least 16
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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INSTRUCTIONS HBEPC NORMAL
Format: PDF
PRODUCTS
| PRODUCT | SIZE | CAT.# |
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| Cryopreserved Human Bronchial Epithelial Cell Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 502K-05a |
| Cryopreserved Bronchial Epithelial Cells (HBEpC), adult: Frozen HBEpC (5x10^5) | 1 Cryovial | 502-05a |
| Cryopreserved Human Bronchial Epithelial Cell with Asthma Total Kit, adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 502ASK-05a |
| Cryopreserved Bronchial Epithelial Cells, Asthma (HBEpC-AS), adult: Frozen HBEpC-AS from donor with Asthma (5x10^5) | 1 Cryovial | 502AS-05a |
| Cryopreserved Human Bronchial Epithelial COPD Cell Total Kit, adult: 5x10^5 Cells (from donor with COPD, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 502COPDK-05a |
| Cryopreserved Bronchial Epithelial Cells, COPD (HBEpC-COPD), adult: Frozen HBEpC-COPD from donor with Chronic Obstructive Pulmonary Disease (5x10^5) | 1 Cryovial | 502COPD-05a |
| Proliferating Bronchial Epithelial Cells (HBEpC), adult: Actively growing, dividing cells in medium | T-25 Flask | 503-25a |
| Proliferating Bronchial Epithelial Cells (HBEpC), adult: Actively growing, dividing cells in medium | T-75 Flask | 503-75a |
| Proliferating Bronchial Epithelial Cells (HBEpC), adult: Actively growing, dividing cells in medium | 24 Well | 503-24Wa |
| Proliferating Bronchial Epithelial Cells (HBEpC), adult: Actively growing, dividing cells in medium | 96 Well | 503-96Wa |
RESOURCES/DOCUMENTS
CELL APPS FLYER EPITHELIAL CELLS
Format: PDF