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BrilliantDye™ Terminator Cycle Sequencing Kit

The BrilliantDye™ Terminator Cycle Sequencing Kits are based on the trusted Sanger Chain Termination method.

Format: The kits are delivered as a 2.5x concentrated ready-reaction premix, fully optimized for a highly flexible chemistry, designed for all kinds of Sequencing applications, including de novo sequencing and resequencing. The kits generate data with uniform peak heights and optimized signal balance to produce long, high-quality reads.

Two Versions Available: The BrilliantDye Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer. The BrilliantDyeTerminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.

Dye Set: The kits are optimized to run with Dye Set E for BrilliantDye Terminator v1.1 and Dye Set Z for BrilliantDye Terminator v3.1.

Increased Performance: By combining the robustness and better mobility of the dye set, with a novel enzyme and optimized dNTP/ddNTP ratios for longer reads, BrilliantDye is the universal Sequencing kit that can be used for all kinds of applications:

  1. Sequencing of (short) PCR products with fast, rapid, or ultra-rapid run modules and High Quality Base Calling for the small fragments.
  2. Sequencing of (long range) PCR products with maximum read lengths with standard or long run modules.
  3. Sequencing of Plasmid DNA with maximum read length.
  4. Sensitive Heterozygote detection with optimized peak heights distribution.

Details
Name Cat# Pack Size RR Seq. Premix 5X Seq. Buffer pGEM control M13 (-21) primer
BrilliantDye v1.1 BRD1-024 24 Rxns 192ul 0.65ml 10ul 10ul
BRD1-100 100 Rxns 800ul 2.0ml 10ul 10ul
BRD1-1000 1000 Rxns 10x800ul 8x2.0ml 50ul 50ul
BRD1-5000 5000 Rxns 2x20ml 2x28ml 50ul 50ul
BrilliantDye v3.1 BRD3-024 24 Rxns 192ul 0.65ml 10ul 10ul
BRD3-100 100 Rxns 800ul 2.0ml 10ul 10ul
BRD3-1000 1000 Rxns 10x800ul 8x2.0ml 50ul 50ul
BRD3-5000 5000 Rxns 2x20ml 2x28ml 50ul 50ul
BRD3-25K 25000 Rxns 10x20ml 10x28ml 50ul 50ul
dGTPBrilliantDye v3.1 BRDG-100 100 Rxns 800ul 2.0ml 10ul 10ul
Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
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Email# customerservice@lifetechindia.com