The BrilliantDye™ Terminator Cycle Sequencing Kits are based on the trusted Sanger Chain Termination method.
Format: The kits are delivered as a 2.5x concentrated ready-reaction premix, fully optimized for a highly flexible chemistry, designed for all kinds of Sequencing applications, including de novo sequencing and resequencing. The kits generate data with uniform peak heights and optimized signal balance to produce long, high-quality reads.
Two Versions Available: The BrilliantDye Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer. The BrilliantDyeTerminator v3.1. Cycle Sequencing Kit is the method of choice for longest reads.
Dye Set: The kits are optimized to run with Dye Set E for BrilliantDye Terminator v1.1 and Dye Set Z for BrilliantDye Terminator v3.1.
Increased Performance: By combining the robustness and better mobility of the dye set, with a novel enzyme and optimized dNTP/ddNTP ratios for longer reads, BrilliantDye is the universal Sequencing kit that can be used for all kinds of applications:
- Sequencing of (short) PCR products with fast, rapid, or ultra-rapid run modules and High Quality Base Calling for the small fragments.
- Sequencing of (long range) PCR products with maximum read lengths with standard or long run modules.
- Sequencing of Plasmid DNA with maximum read length.
- Sensitive Heterozygote detection with optimized peak heights distribution.
|Name||Cat#||Pack Size||RR Seq. Premix||5X Seq. Buffer||pGEM control||M13 (-21) primer|
|BrilliantDye v1.1||BRD1-024||24 Rxns||192ul||0.65ml||10ul||10ul|
|BrilliantDye v3.1||BRD3-024||24 Rxns||192ul||0.65ml||10ul||10ul|
|dGTPBrilliantDye v3.1||BRDG-100||100 Rxns||800ul||2.0ml||10ul||10ul|