 |
Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# customerservice@lifetechindia.com |
Introduction:
flg22 Peptide is a peptide with its sequence derived from the flagellin N-terminus of Pseudomonas sp. and is known to elicit specific innate immune response in plants as well as animals. Amino acid sequence: QRLSTGSRINSAKDDAAGLQIA
P6622 flg22 peptide
| Synonyms: | flagellin peptide (30-51 aa, P. aeruginosa) |
| CAS: | N/A |
| Formula: | C93H162N32O34 |
| Theoretical MW: | 2272.5 g/mol |
Properties
| Form: | Lyophilized powde |
| Appearance: | White to off-white |
| Solubility: | Soluble in sterile water (1 mg/mL) or DMF |
| Application: | Plant Defense and Immunity |
| Storage Temp: | -20°C or below |
| Amino Acid Sequence: | QRLSTGSRINSAKDDAAGLQIA |
| Typical Working Concentration: | 100 pM to 100 nM (Varies with application) |
Application Notes
Peptide sequence derived from the flagellin N-terminus of P. aeruginosa that is known to elicit specific innate immune responses in plants as well as animals. It is considered a PAMP (pathogen associated molecular pattern) by its conserved 22-amino acid sequence. In A. thaliana it leads to activation of MAP (mitogen activated protein) kinases as well as activation of PR (pathogenesis-related) genes.
Dissolve in sterile, deionized water. Store at -20°C or below. Aliquot into multiple tubes to avoid multiple freezethaw events. Note peptides and proteins are all susceptible to binding on the surfaces of plastic and glass tubes and bottles and significant losses can be realized during dilutions near or below 10 g/mL. This is a well-known phenomenon for all peptides and proteins and has been seen specifically in the case of flg22 (Felix et al. 1999). To overcome this we would recommend dilutions below 1.0 mg/mL be performed with an aqueous solution of 0.05M NaCl (S624) and 0.1 mg/mL hydrolyzed casein (C184). Bovine serum albumin (BSA) has often been used in the same capacity as hydrolyzed casein, however we recommend hydrolyzed casein due to its widespread use in plant tissue culture.
References
G. Felix et al. (1999) “Plants have a sensitive perception system for the most conserved domain of bacterial flagellin.” Plant J. Vol. 18(3) pg 265-276. S.T. Chisholm et al. (2006) “Host-Microbe Interactions: Shaping the Evolution of the Plant Immunne Response” Cell Vol 124(4) pg 803-814.