ELISA- Enzyme-Linked Immunosorbent Assay (also called “enzyme immunoassay,” or EIA) is a microtitre plate based quantitative technique in which an antibody is linked to an enzyme (preferably HRP or AP) prior to reaction with the antigen and is then followed by assessment of the enzyme conjugated antibody–antigen reaction upon incubation with the enzyme-specific substrate. The reaction produces a detectable signal as change in the substrate color that could measured at specific wavelength.
There are four major types of ELISA
- Direct ELISA
- Indirect ELISA
- Sandwich ELISA
- Competitive ELISA
Most of plant pathogen diagnostic ELISA kits designed to be highly sensitive and specific. So, it use more than one antibody system which falls under Sandwich ELISA.
There are two types of sandwich ELISA
1. Double antibody sandwich ELISA (DAS- ELISA): In this assay, monoclonal capture antibody (CAB) and polyclonal detection antibody with enzyme conjugate sandwiches the antigen of interest. This is then incubated with substrate that reacts with the enzyme and develop colour. This protocol allows sensitive and specific detection of pathogen/ antigen of interest.
2. Triple antibody sandwich ELISA (TAS- ELISA)/ Compound-ELISA: In this assay, capture antibody (CAB) and detection antibody sandwiches the antigen of interest. This is further incubated with antibody – enzyme conjugate. The enzyme reacts with substrate and develop colour which could be read visually or in ELISA plate reader.
ELISA kits for plant pathogen detection by Agdia are available in three different formats.
PathoScreen® Complete Kit (PSA): Complete ELISA kits for plant pathogen detection that contain all the materials necessary to perform the ELISA test including precoated microtiter ELISA plates, Capture antibody (CAB), conjugated antibodies (ECA), and buffer pack. Kits are available in three sizes: 96, 288 and 480 testwells.
ELISA kits for plant pathogen detection by Agdia are available in three different formats.
Agdia ELISA Set (SRA): Capture antibody (CAB), conjugated antibodies (ECA) and microtitre plates for various antigen detectionAgdia ELISA sets are available in four sizes: 96, 500, 1,000 and 5,000 testwells.
RKA ELISA complete Kit: On customers request, this complete ELISA kit been designed for bulk orders. This kit includes: ELISA plates, Agdia ELISA Set, Positive control, Negative control and Buffer Packs. These kits are popular among customers for diagnosis of Banana virus, Potato virus, Cucurbits virus, Tomato virus etc.
Please check the YouTube videos below to learn more on ELISA procedure.
Click here to see all ELISA test kits available for various plant pathogens.
ImmunoStrip
ImmunoStrip®: The rapid test/ Lateral flow immunostrips- ISK/STX are available for more than 24 pathogens can easily test in the field, with lab-quality results in 30 minutes. Get the answers you need, when you need them!
- Select and Extract Sample: Select sample from plant and insert into the mesh-lined bag. Grind by rubbing the outside of the bag with a blunt object.
- Insert ImmunoStrip: Insert ImmunoStrip Into the extraction bag with the green end facing down. Allow the test to run 30 minutes.
- Interpret Results: If the sample is negative, only the control line will be visible. If the sample is positive, a colored test line will appear below the control line.
ImmunoComb®: These tests are a rapid means of screening crops for the presence of multiple pathogens. ImmunoCombs consist of four ImmunoStrips attached together for ease of testing. ImmunoComb tests require no equipment or expertise to run. Results are obtained in as little as a few minutes making them perfect for use in the field or greenhouse.
Please check the YouTube videos below to learn more on ImmunoStrip procedure.
Click here to see all ImmunoStrip and Immunocomb kits available for various plant pathogens.
AmplifyRP® Isothermal Amplification
Agdia AmplifyRP® comes in two different formats- Acceler8 and XRT.
AmplifyRP® Acceler8® : AmplifyRP® Acceler8® is a rapid RNA amplification and detection platform designed for field-based or laboratory testing of different plant pathogens. It is formulated to allow an end-point detection of amplified products using a lateral flow strip. This lateral flow strip is housed inside an Amplicon Detection Chamber that is specifically designed to prevent the escape of amplified products and thereby also prevent laboratory contamination. AmplifyRP® Acceler8®enables users to rapidly amplify RNA at a single operating temperature using only a crude sample extract. Amplified product is then tested in an Amplicon Detection Chamber where clear "yes / no" results are read visually on a lateral flow strip (much like a pregnancy test). The entire testing process can be completed in as little as 30 minutes.
Please check the YouTube videos below to learn more on AmplifyRP® Acceler8® procedure.
Click here to see all AmplifyRP® Acceler8® tests kits available for various plant pathogens.
AmplifyRP® XRT and XRT+
XRT and XRT+ kits provide real-time isothermal nucleic acid amplification and detection in a portable and easy-to-use format. XRT reaction pellets contain Exonuclease III which will process suitably designed probes during the amplification step and produce a real-time read out much like a real-time PCR. Using AmplifyRP, end-users can obtain the same high level of sensitivity and specificity as PCR but with some distinct advantages.
- Crude sample preparation<
- Ready-to-use amplification reagents
- Pre-optimized and validated
- Real-time results in 30 minutes or less
- Lower equipment cost than PCR / qPCR
Please check the YouTube videos below to learn more on AmplifyRP® XRT procedure.
Click here to see all AmplifyRP® XRT and XRT+ kits available.
AmpliFire® Isothermal Fluorometer
AmpliFire is a portable, battery-powered, isothermal fluorometer that performs amplification and detection in as little as 10 minutes when paired with AmplifyRP XRT and XRT+ chemistry. Featuring an intuitive touch screen interface, integrated analysis software, and barcode scanning for protocol setup, the AmpliFire offers the ultimate ease of use!
Key Features
- Easy portability enabled by small size and rechargeable battery that lasts up to six hours
- Up to eight samples per run
- Easy to use, full-color, touch screen interface
- Ten second scan time across three fluorescence channels
- Supports multiple isothermal chemistries
Benefits
- Use in lab or field settings
- Crude sample preparation
- Rapid sample to answer functionality
- Light weight and small footprint
- Intuitive software workflow
Software and Data Analysis
- Integrated barcode scanner automatically loads protocols
- Amplification curves displayed in real time
- Positive, negative, and indeterminate calls made automatically
- Supports multiple calling algorithms
- Detailed data export available in CSV format
Please check the YouTube videos below to learn more on AmpliFire® Isothermal Fluorometer operating procedure.
Click here to learn more about AmpliFire® Isothermal Fluorometer.
