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Schenk & Hildebrandt Modified Basal Medium w/ Vitamins & Sucrose

  • Product ID: S813

Introduction:

Contains the macro- and micronutrients and vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose.

S813 Schenk & Hildebrandt Modified Basal Medium Contains Vitamins and 10 g/L Sucrose


Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 14.21g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose. pH = 3.75 – 4.75
Formula (mg/L)
Ammonium Phosphate, Monobasic  300
Boric Acid 5
Calcium Chloride, Anhydrous 151
Cobalt Chloride.6H2O 0.1
Cupric Sulfate.5H2O 0.2
Na2 EDTA.2H2O 20
Ferrous Sulfate.7H2O  15
Magnesium Sulfate, Anhydrous 195.4
Manganese Sulfate.H2O  10
Molybdic Acid (Sodium Salt).2H2O  0.1
Potassium Iodide  1
Potassium Nitrate 2500
Zinc Sulfate.7H2O 1
myo-Inosito  1000
Nicotinic Acid (Free Acid)  5
Pyridoxine•HCI  0.5
Sucrose  10,000
Thiamine•HCl  5

Application Notes
Plant Tissue Culture Tested


References
Schenk, RU & AC Hildebrandt. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Can. J. Bot. 50: 199-204.

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