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Introduction:
Contains the ½ macro- and ½ micronutrients and ½ the vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose.
S808 Schenk & Hildebrandt Modified Basal Medium (1/2x and 10 g/L Sucrose)
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 12.10 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the ½ macro- and ½ micronutrients and ½ the vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose. pH = 4.0 – 5.0 |
Properties
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 12.10 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the ½ macro- and ½ micronutrients and ½ the vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose. pH = 4.0 – 5.0 |
| Formula (mg/L) | |
|---|---|
| Ammonium Phosphate, Monobasic | 150 |
| Boric Acid | 2.5 |
| Calcium Chloride, Anhydrous | 75.5 |
| Cobalt Chloride.6H2O | 0.05 |
| Cupric Sulfate.5H2O | 0.1 |
| Na2 EDTA.2H2O | 10 |
| Ferrous Sulfate.7H2O | 7.5 |
| Magnesium Sulfate, Anhydrous | 97.7 |
| Manganese Sulfate.H2O | 5 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.05 |
| Potassium Iodide | 0.5 |
| Potassium Nitrate | 1250 |
| Zinc Sulfate.7H2O | 0.5 |
| myo-Inositol | 500 |
| Nicotinic Acid (Free Acid) | 2.5 |
| Pyridoxine.HCl | 0.25 |
| Sucrose | 10,000 |
| Thiamine.HCl | 2.5 |
Application Notes
Plant Tissue Culture Tested
References
Schenk, RU and AC Hildebrandt. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Can. J. Bot. 50: 199-204