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Introduction:
Staba Modified Vitamin Solution (100x) contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-Inositol was reduced from 2000 mg/L to 100 mg/L. The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus (Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus). myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolve it. Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.
S743 Staba Modified Vitamin Solution (100x)
Properties
| Form: | Liquid |
| Appearance: | Clear Solution |
| Application: | Plant Tissue Culture |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 1 mL/ L of medium |
| Storage Temp: | -20° C |
| Storage Temp of Stock Solution: | N/A |
| Other Notes: | Contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-inositol was reduced from 2000 mg/L to 100 mg/L. Myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolved it. |
| Formula (mg/L) | |
|---|---|
| ?-Aminobenzoic Acid | 50 |
| D-Biotin | 100 |
| Calcium Pantothenate | 100 |
| Choline Chloride | 100 |
| Cyanocobalamin | 0.15 |
| Folic Acid | 50 |
| myo-Inositol | 10,000 |
| Niacinamide | 200 |
| Pyridoxine HCl | 200 |
| Riboflavin | 50 |
| Thiamine HCl | 100 |
Application Notes
Plant Tissue Culture Tested
The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have
been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus
(Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus).
References
Staba, JE. 1969. Plant tissue culture as a technique for the phytochemist. Recent Adv. in Phytochem., 2:80.
Skirvin, RM and MC Chu. 1979. In vitro culture of 'Forever Yours' rose. HortSci., 14: 608-610. (Revised 5/2007)