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Staba Modified Vitamin Solution (100x)

  • Product ID: S743

Introduction:

Staba Modified Vitamin Solution (100x) contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-Inositol was reduced from 2000 mg/L to 100 mg/L. The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus (Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus). myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolve it. Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.

S743 Staba Modified Vitamin Solution (100x)

Properties

Form: Liquid
Appearance: Clear Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working Concentration: 1 mL/ L of medium
Storage Temp: -20° C
Storage Temp of Stock Solution: N/A
Other Notes: Contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-inositol was reduced from 2000 mg/L to 100 mg/L.
Myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolved it.

Formula (mg/L)
?-Aminobenzoic Acid 50
D-Biotin 100
Calcium Pantothenate 100
Choline Chloride 100
Cyanocobalamin 0.15
Folic Acid 50
myo-Inositol 10,000
Niacinamide 200
Pyridoxine HCl 200
Riboflavin 50
Thiamine HCl 100

Application Notes
Plant Tissue Culture Tested
The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus (Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus).


References
Staba, JE. 1969. Plant tissue culture as a technique for the phytochemist. Recent Adv. in Phytochem., 2:80.
Skirvin, RM and MC Chu. 1979. In vitro culture of 'Forever Yours' rose. HortSci., 14: 608-610. (Revised 5/2007)

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