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Introduction:
This medium contains high calcium level which may inhibit many gellan gums from completely melting during autoclaving. For this reason the use of Gellan Gum and Agargellan with this medium is not recommended as a clear, firm gel may not be obtained.
Contains the macro- and micronutrients as described by Quoirin & Lepoivre (1977) and Quoirin, et al. (1977).
Q673 Quoirin & Lepoivre Basal Salt Mixture
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 3.56g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Quoirin and Lepoivre (1977) and Quoirin et al. (1977) pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 400 |
| Boric Acid | 6.2 |
| Calcium Nitrate | 833.77 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 175.79 |
| Manganese Sulfate.H2O | 0.76 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Iodide | 0.08 |
| Potassium Nitrate | 1800 |
| Potassium Phosphate, Monobasic | 270 |
| Zinc Sulfate.7H2O | 8.6 |
Application Notes
Plant Tissue Culture Tested Plant species: Prunus spp.
References
Quoirin, M. and P Lepoivre. 1977. Improved medium for in vitro culture of Prunus sp. Acta. Hort. 78:437-442. Quoirin, M, et al. 1977. C. R. Res. Sta. Cult. Fruit Mar., Gembloux 93.