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Orchid Seed Sowing Medium II

  • Product ID: P727

Introduction:

This medium is a modification of P723 Orchid Seed Sowing Medium, except charcoal has been eliminated, and is best suited for species that do not produce phenolics. This medium may be used for some terrestrial orchid species by preparing the medium at ½ strength and supplementing it with 50 ml of coconut water per liter.

P727 Orchid Seed Sowing Medium Contains Agar, Without Charcoal




Properties

Form: Powder
Appearance: Off-white to Cream Powde
Application: Orchid Culture
Solubility: Hot Water
Typical Working Concentration: 31.74 g/L
Storage Temp: 2-8° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: The pH of this medium should be adjusted prior to sterilization.
Formula (mg/L)
Ammonium Nitrate 
Boric Acid  1.65
Calcium Chloride, Anhydrous  83
Cobalt Chloride.6H2O  0.0063
Cupric Sulfate.5H2O  0.0063
Na2EDTA.2H2O  18.65
Ferrous Sulfate.7H2O  13.93
Magnesium Sulfate,Anhydrous  75.18
Manganese Sulfate.H2O  4.23
Molybdic Acid, Sodium Salt.2H2O  0.0625
Potassium Iodide  0.2075
Potassium Nitrate  475
Potassium Phosphate, Monobasic 42.5
Zinc Sulfate.7H2O 2.65
Agar 8000
MES (Free Acid) 500
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Peptone from Meat 2000
Pyridoxine.HCl 1
Sucrose  20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Phalaenopsis, Epiphytic orchids
This medium is a modification of P723 Orchid Seed Sowing Medium, ex cept charcoal has been eliminated, and is best suited for s pecies that do not produce phenolics.
This medium may be used for some terrestrial orchid species by preparing the medium at ½ strength and supplementing it with 50 ml of coconut water per liter. Approximately 4 g/L of agar should also be added. (Revised 12/2012)

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