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Introduction:
This medium is a modification of our Orchid Maintenance/Replate Medium (P748). It lacks a gelling agent to permit its use in a liquid culture system. This medium contains banana powder to promote growth and rooting. The pH of this medium should be adjusted after adding the gelling agent and prior to sterilization.
O156 Orchid Maintenance Medium Contains Banana and Charcoal, Without Agar
Properties
| Form: | Powder |
| Appearance: | Gray powder |
| Application: | Orchid Cultur |
| Solubility: | Partially Soluble in Water |
| Typical Working Concentration: | 57.31 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | pH = 4.5 - 5.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 825 |
| Boric Acid | 3.11 |
| Calcium Chloride, Anhydrous | 166 |
| Cobalt Chloride.6H2O | 0.0125 |
| Cupric Sulfate.5H2O | 0.0125 |
| Na2EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 90.35 |
| Manganese Sulfate.H2O | 8.45 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.125 |
| Potassium Iodide | 0.415 |
| Potassium Nitrate | 950 |
| Potassium Phosphate, Monobasic | 85 |
| Zinc Sulfate.7H2O | 5.3 |
| Activated Charcoal | 2000 |
| Banana Powder | 30000 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Peptone from Meat | 2000 |
| Pyridoxine.HCl | 1 |
| Sucrose | 20000 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Epiphytic orchid species
This medium is a modification of our Orchid
Maintenance/Replate Medium
(P748). It lacks a
gelling agent to permit its use in a liquid cultur
e system. This medium contains banana powder
to promote growth and rooting.
The pH of this medium s
hould be adjusted after adding the
gelling agent and prior to sterilization.