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Introduction:
Contains the macro- and micronutrients, and vitamins as described by Nitsch and Nitsch (1969).
This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
N616 Nitsch & Nitsch Basal Medium w/Vitamins
Properties
| Properties | |
|---|---|
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 2.21g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Nitsch and Nitsch (1969) |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 720 |
| Boric Acid | 10 |
| Calcium Chloride, Anhydrous | 166 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 90.372 |
| Manganese Sulfate.H2O | 18.9 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Nitrate | 950 |
| Potassium Phosphate, Monobasic | 68 |
| Zinc Sulfate.7H2O | 10 |
| D-Biotin | 0.05 |
| Folic Acid | 0.5 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine.HCl | 0.5 |
| Thiamine.HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested
Plant species: Nicotiana (tobacco) This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.