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Nitsch & Nitsch Basal Salt Mixture

  • Product ID: N613

Introduction:

Contains the macro- and micronutrients as described by Nitsch and Nitsch (1969).
This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.

N613 Nitsch & Nitsch Basal Salt Mixture

Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 2.10g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Nitsch and Nitsch (1969) pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate  720
Boric Acid  10
Calcium Chloride, Anhydrous  166
Cupric Sulfate.5H2O  0.025
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O  27.8
Magnesium Sulfate, Anhydrous  90.372
Manganese Sulfate.H2O  18.9
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Nitrate  950
Potassium Phosphate, Monobasic  68
Zinc Sulfate.7H2O 10

Application Notes
Plant Tissue Culture Tested Plant species: Nicotiana (tobacco) This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.


References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87

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