Enquiry E-CATALOGUE

NB Basal Medium

  • Product ID: N492

Introduction:

Modified Chu/Gamborg basal medium. Contains the macronutrients described by Chu (1975) & the micronutrients described by Gamborg, et al. (1968).

N492 NB Basal Medium Modified Chu/ Gamborg Basal Medium


Synonym: WPM Basal Salts

Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.10g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Sulfate 463
Boric Acid  3
Calcium Chloride, Anhydrous  125.33
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O  27.8
Magnesium Sulfate, Anhydrous  90.37
Manganese Sulfate.H2O 10
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Iodide  0.75
Potassium Nitrate  2830
Potassium Phosphate, Monobasic  400
Zinc Sulfate.7H2O 2
myo-Inositol  100
Nicotinic Acid (Free Acid) 1
Pyridoxine.HCl 1
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested


References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.


Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.

Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com