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Introduction:
Modified Chu/Gamborg basal medium. Contains the macronutrients described by Chu (1975) & the micronutrients described by Gamborg, et al. (1968).
N492 NB Basal Medium Modified Chu/ Gamborg Basal Medium
Synonym: WPM Basal Salts
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 4.10g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Sulfate | 463 |
| Boric Acid | 3 |
| Calcium Chloride, Anhydrous | 125.33 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 90.37 |
| Manganese Sulfate.H2O | 10 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Iodide | 0.75 |
| Potassium Nitrate | 2830 |
| Potassium Phosphate, Monobasic | 400 |
| Zinc Sulfate.7H2O | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Pyridoxine.HCl | 1 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.