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NLN Basal Medium

  • Product ID: N479

Introduction:

Contains the macro- and micronutrients, and organic constituents as described by Lichter (1982). Without sucrose.
This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L Calcium Nitrate tetrahydrate as described in the original formulation.

N479 NLN Basal Medium

Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 1.77g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and organic constituents as described by Lichter (1982).
Formula (mg/L)
Boric Acid 10
Calcium Nitrate (dried)  347
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Ferric Sodium EDTA  36.7
Magnesium Sulfate, Anhydrous  61
Manganese Sulfate.H2O  18.95
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Nitrate 125
Potassium Phosphate, Monobasic  125
Zinc Sulfate.7H2O  10
D-Biotin  0.05
Folic Acid 0.5
L-Glutamine 800
Glutathione 30
Glycine (Free Base)  2
myo-Inositol  100
Nicotinic Acid (Free Acid)  5
Pyridoxine.HCl 0.5
L-Serine 100
Thiamine.HCl  0.5

Application Notes
Plant Tissue Culture Tested Plant species: Brassica napus This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L calcium nitrate tetrahydrate as described in the original formulation.


References
Lichter, R. 1981. Z. Pflanzenphysiol. 103: 119. Lichter, R. 1982. Z. Pflanzenphysiol. 105: 427

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