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Murashige Begonia Multiplication Medium with Ferric Sodium EDTA

  • Product ID: M701

Introduction:

M701 Murashige & Skoog (MS) Modified Basal Medium


Synonym: Murashige Begonia Multiplication Medium

Properties

Form: Fine to Fluffy Powder
Appearance: White to Off-white Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.44g/L
Storage Temp: 2-8°
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exception: contains Ferric Sodium EDTA in place of Disodium EDTA and Ferrous Sulfate. Modified with the addition of (mg/L): 10.0 2iP, 1.0 IAA, and 0.4 Thiamine in place of MS vitamins
Formula (mg/L)
Ammonium Nitrate 1650
 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Adenine Hemisulfate 30
6-γ,γ-Dimethylallylaminopurine (2iP) 10
Indole-3-acetic Acid 1.0
myo-Inositol 100
Thiamine .HCl   0.4

Application Notes
Plant Tissue Culture Tested
Plant species: Begonia


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497

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