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Murashige & Skoog Basal Medium with Vitamins, Sucrose, & Gelzan

  • Product ID: M5825

Introduction:

Contains the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962). Modified to contain 30 g/L D-Sucrose and 2 g/L Gelzan.

M5825 Murashige & Skoog (MS) Basal Medium w/Sucrose & Gelzan


Synonym: MS Medium w/ Vitamin, Sucrose & Gelzan

Properties

Form: Fine Powder
Appearance: Tan Powder
Application: Plant Tissue Culture
Solubility: Partially Soluble Water
Typical Working Concentration: 34.43 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Approximate unadjusted pH = 3.5-5.0
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate  , Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Gelzan 2000.0
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Glycine(Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
 Pyridoxine .HCl 0.5
Sucrose 30,000
Thiamine•HCl 0.1

Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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