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Introduction:
Contains the macro- & micronutrients as described by Murashige & Skoog (1962), and as Modified by Van Der Salm t.m.p. et al. (1994).
M5541 Murashige & Skoog (MS) Basal Salt Mixture Van der Salm Modification
Properties
| Form: | Fine Powder |
| Appearance: | Light Burgandy Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 4.36 g/L |
| Storage Temp: 2-8° | |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the iron source as described by Van der Salm et al. (1994). Unadjusted pH = Approx. 3.5-4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| FeNa-EDDHA | 96 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
Application Notes
Plant Tissue Culture Tested
Plant species: Originally published for use on the rose rootstock cv 'Moneyway' due to chlorosis
in shoots cultured on the standard MS formula.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
Van der Salm, TMP, CJG Van der Toorn, CH Hänisch ten Cate, LAM Dubois, DP De Vries, and HJM Dons. 1994. Importance of the iron chelate formula for micropropagation of Rosa Hybrida L. 'Moneyway'. Plant Cell Tiss. and Organ Cult. 37:73-77.