Enquiry E-CATALOGUE

Murashige & Skoog Basal Salt Mixture, van der Salm Modification

  • Product ID: M5541

Introduction:

Contains the macro- & micronutrients as described by Murashige & Skoog (1962), and as Modified by Van Der Salm t.m.p. et al. (1994).

M5541 Murashige & Skoog (MS) Basal Salt Mixture Van der Salm Modification

Properties

Form: Fine Powder
Appearance: Light Burgandy Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.36 g/L
Storage Temp: 2-8°  
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the iron source as described by Van der Salm et al. (1994). Unadjusted pH = Approx. 3.5-4.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
FeNa-EDDHA 96
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6

Application Notes
Plant Tissue Culture Tested
Plant species: Originally published for use on the rose rootstock cv 'Moneyway' due to chlorosis in shoots cultured on the standard MS formula.


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.


Van der Salm, TMP, CJG Van der Toorn, CH Hänisch ten Cate, LAM Dubois, DP De Vries, and HJM Dons. 1994. Importance of the iron chelate formula for micropropagation of Rosa Hybrida L. 'Moneyway'. Plant Cell Tiss. and Organ Cult. 37:73-77.

Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com