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Murashige & Skoog Medium with Vitamins and 30 g/L Sucrose (1X)

  • Product ID: M5530

Introduction:

Sterile filtered liquid solution containing the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962), then modified to contain 15 g/L sucrose and 15 g/L glucose.

M5530 Murashige & Skoog (MS) Basal Medium Solution w/ Vitamins& Sucrose (1X)

Synonyms: MS Medium

Properties

Form: Liquid
Appearance: Clear, Colorless to Light Yellow
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working Concentration: 1x
Storage Temp: 2-8°C
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962).
pH = 3.5-4.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine.HCl 0.5
Thiamine.HCl 0.1
Sucrose 30,000

Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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