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Introduction:
Sterile filtered liquid solution containing the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962), then modified to contain 15 g/L sucrose and 15 g/L glucose.
M5530 Murashige & Skoog (MS) Basal Medium Solution w/ Vitamins& Sucrose (1X)
| Synonyms: | MS Medium |
Properties
| Form: | Liquid |
| Appearance: | Clear, Colorless to Light Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 1x |
| Storage Temp: | 2-8°C |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). pH = 3.5-4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Pyridoxine.HCl | 0.5 |
| Thiamine.HCl | 0.1 |
| Sucrose | 30,000 |
Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with
tobacco tissue cultures. Physiol. Plant. 15: 473-497.