M551 Malmgren Modified Terrestrial Orchid Medium Without Sucrose
Properties
Form:
Powder
Appearance:
Gray powder
Application:
Orchid Culture
Solubility:
Water
Typical Working Concentration:
28.84 g/L
Storage Temp:
2-8° C
Storage Temp of Stock Solution:
Preparation of concentrated solutions
is not recommended as insoluble
precipitates may form.
Other Notes:
Contains the macro- and micronutrients, agar,
and organic constituents as
described by Malmgren (1996)
pH = 3.75 - 4.75
Formula (mg/L)
Calcium Phosphate, Tribasic
75
Na2 EDTA.2H2O
37.26
Ferrous Sulfate.7H2O
27.8
Magnesium Sulfate, Anhydrous
97.69
Manganese Sulfate.H2O
1.54
Potassium Phosphate, Monobasic
75
Activated Charcoal
1000
Agar
7000
D-Biotin
0.05
Casein, Enzymatic Hydrolysate
400
Folic Acid
0.5
Glycine (Free Base)
2
myo-Inositol
100
Nicotinic Acid (Free Acid)
5
Pineapple Powder
20,000
Pyridoxine.HCl
5
Thiamine.HCl
10
Application Notes
Plant Tissue Culture Tested
Plant species: Terrestrial or
chids including Paphiopedilum,
Cypripedium, and Phragmipedium
This medium is a good seed sowing medium for many
terrestrial orchid species. Originally
developed for Cypripedium, this medium is al
so used for germination of Phragmipedium and
Paphiopedilum. Germination may be enhanced by in
cluding 50 ml of Coconut Water (C195) per
liter of medium.
References
Malmgren, S. 1996. In: C. Allen, Editor, North Americ
an Native Terrestrial Orchids.
Propagation and Production C
onf. Proc., pp 63-71.