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Murashige & Skoog Modified Medium (Arabidopsis Culture Medium) with 2,4-D and Kinetin

  • Product ID: M550

Introduction:

Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 2.0 2,4-D, 0.05 Kinetin, and sucrose.

M550 Murashige & Skoog (MS)Modified Medium Arabidopsis Culture Medium

Properties

Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 24.44g/L
Storage Temp: 2-8°
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 2.0 2,4-D, 0.05 Kinetin, and sucrose
Formula (mg/L)
Ammonium Nitrate 1650
 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
2,4-Dichlorophenoxyacetic Acid 2.0
Kinetin 0.05
myo-Inositol 100
Nicotinic Acid (Free Acid) 1.0
Pyridoxine•HCl  1.0
Sucrose 20,000
Thiamine .HCl   0.4

Application Notes
Plant Tissue Culture Tested
Plant species: Arabidopsis thaliana
This medium was developed for the culture of Arabidopsis callus and cell suspension cultures


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497

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