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Murashige & Skoog Modified Basal Medium with Sodium Phosphate and Ferric Sodium EDTA

  • Product ID: M541

Introduction:

Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exception: Without Potassium Phosphate Monobasic. Also contains (mg/mL): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, 1000 Casein, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.

M541 Murashige & Skoog (MS)Modified Basal Medium


Properties

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 5.69 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exceptions: Potassium Phosphate Monobasic is omitted, Sodium Phosphate Monobasic is added, and Ferrous Sulfate and Disodium EDTA are replaced with Ferric Sodium EDTA. Approx. pH = 4.75 ± 0.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate•H2O 16.9
Molybdic Acid (Sodium Salt)•2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Sodium Phosphate Monobasic 300
Zinc Sulfate•7H2O 8.6
Adenine Hemisulfate 150
Casein, Enzymatic Hydrolysate 1000
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pyridoxine•HCl 1
Thiamine•HCl 0.5

Application Notes
Plant Tissue Culture Tested

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497

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