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Murashige Modified Basal Multiplication Medium

  • Product ID: M536

Introduction:

Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Also contains (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, and 0.4 Thiamine.

M536 Murashige Modified Multiplication Basal Medium


Properties

Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.68 g/L
Storage Temp: 2-8°
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Also contains (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, and 0.4 Thiamine. pH = 3.25-4.25
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
 Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
 Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
 Molybdic Acid (Sodium Salt).2H2O 0.25
 Potassium Iodide  0.83
 Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Sodium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Adenine Hemisulfate 80
Glycine (Free Base) 2
 myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
 Pyridoxine .HCl 0.5
Thiamine .HCl 0.4

Application Notes
Plant Tissue Culture Tested


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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