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Murashige & Skoog Modified Basal Medium with LS Vitamins and Adenine Sulphate

  • Product ID: M535

Introduction:

Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains 80 mg/L Adenine Hemisulfate.

M535 Murashige & Skoog (MS) Modified Basal Medium


Properties

Form: Fine to Fluffy Powder
Appearance: White to Off-white Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.51 g/L
Storage Temp: 2-8°
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains 80 mg/L Adenine Hemisulfate. pH = 3.25 – 4.25
Formula (mg/L)
Ammonium Nitrate 1650
 Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Adenine Hemisulfate 80
myo-Inositol 100
Thiamine .HCl   0.4

Application Notes
Plant Tissue Culture Tested
Plant species: Spathiphyllum, ferns, Ficus spp
This medium is also used for the micropropagation of numerous additional tropical foliage plants


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497

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