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Murashige & Skoog Modified Basal Salt Mixture (No Nitrogen)

  • Product ID: M531

Introduction:

Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: No Ammonium Nitrate (A300) or Potassium Nitrate (P100).

M531 Murashige & Skoog (MS) Modified Basal Salt Mixture (No Nitrogen)


Synonym: Modified MS Medium

Properties

Form: Fine to Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 0.78 g/L
Storage Temp: 2-8 °C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: No Ammonium Nitrate or Potassium Nitrate.
Formula (mg/L)
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Iodide 0.83
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6

Application Notes
Plant Tissue Culture Tested Nitrogen must be supplemented to avoid nitrogen deficiency.


References
Murashige, T and F Skoog. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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