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Murashige Modified Multiplication Medium

  • Product ID: M527

Introduction:

This medium may be sold as Murashige & Skoog (MS) Shoot Multiplication Medium A by some media suppliers.
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains (mg/L): 170 Sodium Phosphate monobasic, 80 Adenine Hemisulfate, 30.0 2iP, 0.3 IAA, and FeNa-EDTA in place of Ferrous Sulfate and Na2-EDTA.

M527 Murashige Modified Multiplication Basal Medium


Synonym: Murashige & Skoog (MS) Shoot Multiplication Medium A

Properties

Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.68 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30.0 2iP, 0.3 IAA, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.
Formula (mg/L)
Ammonium Nitrate  1650
Boric Acid  6.2
Calcium Chloride, Anhydrous  333
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Ferric Sodium EDTA  36.7
Magnesium Sulfate, Anhydrous  181
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt). 2H2O  0.25
Potassium Iodide  0.83
Potassium Nitrate  1900
Potassium Phosphate, Monobasic  170
Sodium Phosphate, Monobasic  170
Zinc Sulfate.7H2O  8.6
Adenine Hemisulfate  80
6-?,?-Dimethylallylaminopurine (2iP)  30
Indoleacetic Acid  0.3
myo-Inositol  100
Thiamine.HCl  0.4

Application Notes
Plant Tissue Culture Tested


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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