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Murashige & Skoog Modified BC Potato Medium

  • Product ID: M516

Introduction:

Contains the macro & micronutrients described by Murashige & Skoog, & vitamins described by Gamborg, et al (1966).

M516 Murashige & Skoog Modified BC Potato Basal Medium




Properties

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.44g/L
Storage Temp: 2-8°
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962). Contains Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA. Also contains Kinetin and the vitamins cited below. pH = 4.25 – 5.25
Formula (mg/L)
Ammonium Nitrate 1650
 Boric Acid 6.2
Calcium Chloride, Anhydrous 333
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate•7H2O 8.6
Glycine 2.0
Kinetin 0.04
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine•HCl 0.5
Thiamine•HCl 0.4

Application Notes
Plant Tissue Culture Tested
Plant species: Potato


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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