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Murashige Gerbera Multiplication Medium

  • Product ID: M509

Introduction:

Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 85 Sodium Phosphate monobasic, 80 Adenine Hemisulfate, 10.0 Kinetin, 0.5 IAA, 100 L-tyrosine, and FeNa-EDTA in place of Ferrous Sulfate and Na2-EDTA.

M509 Murashige Modified Gerbera Multiplication Basal Medium




Properties

Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.72 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 85 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 10.0 Kinetin, 0.5 IAA, 100 L-Tyrosine, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA
Formula (mg/L)
Ammonium Nitrate 1650
 Boric Acid 6.2
Calcium Chloride, Anhydrous 333
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
 Ferric Sodium EDTA  36.7
 Magnesium Sulfate, Anhydrous  181
 Manganese Sulfate.H2O 16.9
 Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic  170
 Sodium Phosphate, Monobasic 85
Zinc Sulfate.7H2O  8.6
 Adenine Hemisulfate 80
 Kinetin 10.0
Indoleacetic Acid  0.5
 Myo-Inositol 100
Nicotinic Acid (Free Acid)  10.0
 Pyridoxine.HCl 1.0
Thiamine.HCl 30
 L-Tyrosine 100

Application Notes
Plant Tissue Culture Tested. Plant species: Gerbera Daisies


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127

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