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Murashige Modified Cattleya Orchid Multiplication Medium

  • Product ID: M507

Introduction:

Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins.
Contains 20 g/L Sucrose. Also contains: 150 mg/L Citric Acid, 0.3 mg/L IAA, 1.75 mg/L IBA, 1.75 mg/L NAA, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.

M507 Murashige Cattleya Orchid Multiplication Medium




Properties

Form: Powder
Appearance: White to Off-white Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 24.57 g/L
Storage Temp: 2-8° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962).
pH = 2.75 - 3.75
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 333
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Citric Acid (Free Acid) Anhydrous 150
Glycine (Free Base) 2
Indole-3-acetic Acid 0.3
Indole-3-butyric Acid 1.75
myo-Inositol 100
a-Naphthaleneacetic Acid 1.75
Nicotinic Acid (Free Acid) 0.5
Pyridoxine.HCl 0.5
Sucrose 20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Cattleya and other epiphytic orchids.


References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

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