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Musa (Banana) Multiplication Medium

  • Product ID: M462

Introduction:

Contains the macro- and micronutrients as described by Murashige and Skoog (1962).
IITA formulation as described by Vuylsteke (1989) (International Institute for Tropical Agriculture).

M462 Musa (Banana) Multiplication Medium IITA Formulation*




Properties

Form: Powder
Appearance: White to Off-White
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water.
Typical Working Concentration: 36.36 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Formulation as described by Vuylsteke (1989).
pH = 3.75 - 4.75
*International Institute for Tropical Agriculture
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Na2EDTA.2H2O  37.26
Ferrous Sulfate  27.85
Magnesium Sulfate, Anhydrous  180.74
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt).2H2O  0.25
Potassium Iodide  0.83
Potassium Nitrate  1900
Potassium Phosphate Monobasic  170
Zinc Sulfate.7H2O  8.6
L-Ascorbic Acid  20
6-Benzylaminopurine (BA)  4.5
Gellan Gum - Biotech Grade  2000
Glycine  2
Indole-3-acetic Acid  0.175
Nicotinic Acid (Free Acid)  0.5
Pyridoxine.HCl  0.5
Sucrose  30,000
Thiamine.HCl  0.4

Application Notes
Plant Tissue Culture Tested


References
Vuylsteke D (1989) Shoot-tip culture for the propagation, conservation, and exchange of Musa germplasm. Practical manuals for handling crop germplasm in vitro. 2. Rome: International Board for Plant Genetic Resources.

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