Enquiry E-CATALOGUE

Linsmaier & Skoog Basal Medium

  • Product ID: L689

Introduction:

Contains the macro- and micronutrients, & vitamins as described by Linsmaier & Skoog (1964).
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This is a subsequent optimization of the medium developed by Murashige and Skoog. Linsmaier's research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine-HCI was optimized from 0.1 to 0.4 mg/L. Linsmaier also noted that folic acid, ρ-aminobenzoic acid, L-glutamine,and ascorbic acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine-HCI and myo-Inositol.

H




Properties

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 3.9g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins and organic supplements as described by Kao and Michayluk (1975).

Formula (mg/L)
Ammonium Nitrate 600
Boric Acid  3
Calcium Chloride, Anhydrous 453
Cobalt Chloride·6H2O  0.025
Cupric Sulfate·5H2O  0.025
Na2 EDTA·2H2O  37.26
Ferrous Sulfate·7H2O  27.85
Magnesium Sulfate, Anhydrous  146.55
Manganese Sulfate·H2O 10
Molybdic Acid (Sodium Salt)·2H2O 0.25
Potassium Chloride 300
Potassium Iodide 0.75
Potassium Nitrate 1900
Potassium Phosphate, Monobasic  170
Zinc Sulfate·7H2O  2
p-Aminobenzoic Acid 0.02
L-Ascorbic Acid  2
D-Biotin  0.01
D-Calcium Pantothenate 1
Choline Chloride  1
Citric Acid (Free Acid) Anhydrous  40
Cyanocobalamin (Vitamin B12) 0.02
Folic Acid  0.4
Fumaric Acid  40
DL-Malic Acid 40
myo-Inositol  100
Niacinamide  1
Pyridoxine·HCl 1
Pyruvic Acid 20
Riboflavin  0.2
Thiamine·HCl  1
Vitamin A  0.01
Vitamin D3 0.01

Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978); Daucus carota (Grzebelus et al, 2012).


This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.


References
Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332. Grzebelus E, M Szklarczyk & R Baranski (2012) An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot.
Plant Cell Tiss Organ Cult 109:101-109. Kao, KN and MR Michayluk (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.

Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com