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Litvay Basal Salt Mixture

  • Product ID: L546

Introduction:

Contains the macro- and micronutrients as described by Litvay et al. (1981).
Litvay et al. (1981) originally developed the medium to culture both juvenile and mature tissues of Douglas-fir (Pseudotsuga menziesii) and Loblolly pine (Pinus taeda). The original formulation contained 30 g/L sucrose and the following vitamins (mg/L): 100 myo-Inositol, 0.5 Nicotinic Acid, 0.1 Pyridoxine-HCl, and 0.1 Thiamine-HCl.

L546 Litvay Basal Salt Mixture


Synonym: LM Basal Salt Mixture

Properties

Form: Fine Powder
Appearance: Off-white to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 4.95g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Litvay et al. (1981).

Formula (mg/L)
Ammonium Nitrate  1650
Boric Acid  31
Calcium Chloride, Anhydrous  16.61
Cobalt Chloride.6H2O  0.125
Cupric Sulfate.5H2O  0.5
Ferric Sodium EDTA  36.7
Magnesium Sulfate, Anhydrous  903.38
Manganese Sulfate.H2O  21
Molybdic Acid (Sodium Salt). 2H2O 1.25
Potassium Iodide  4.15
Potassium Nitrate  1900
Potassium Phosphate, Monobasic  340
Zinc Sulfate.7H2O 43

Application Notes
Plant species: Douglas-fir, Loblolly Pine, Carrot Litvay et al.
(1981) originally developed the medium to culture both juvenile and mature tissues of Douglas-fir (Pseudotsuga menziesii) and Loblolly pine (Pinus taeda). The original formulation contained 30 g/L sucrose and the following vitamins (mg/L): 100 myo-Inositol, 0.5 Nicotinic Acid, 0.1 Pyridoxine•HCl and 0.1 Thiamine•HCl.
This medium was later used to study embryogenesis in wild carrot (Daucus carota L.) suspension cells (Litvay et al, 1985).

References
Litvay, JD, MA Johnson, DC Verma, D Einspahr, K Weyrauch. 1981. Conifer suspension culture medium development using analytical data from developing seeds. Inst.
Paper Chemistry, IPC Tech Paper Ser No 115. Appleton, WI. Litvay, JD, DC Verma, MA Johnson. 1985. Influence of loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep. 4:325.

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