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Introduction:
Contains the macro- & micronutrients, and vitamins as described by Linsmaier & Skoog (1964). pH adjusted and buffered.
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR's, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuations during media preparation and culture.
L477 Linsmaier & Skoog Modified Basal Medium pH Adjusted and Buffered
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 5.53 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965). pH = 5.25 – 6.25 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Hydroxide | 100 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most
circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuations during media preparation and culture.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.