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Linsmaier & Skoog Modified Basal Medium w/ Sucrose

  • Product ID: L473

Introduction:

Contains the macro- & micronutrients, and vitamins as described by Linsmaier & Skoog (1964). pH adjusted and buffered.
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR's, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuations during media preparation and culture.

L473 Linsmaier & Skoog Modified Basal Medium w/ 30 g/L Sucrose pH Adjusted and Buffered


Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)

Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 35.53g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965).
Formula (mg/L)
Ammonium Nitrate  1650
Boric Acid  6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Na2 EDTA.2H2O  37.26
Ferrous Sulfate.7H2O  27.8
Magnesium Sulfate 180.7
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Hydroxide  100
Potassium Iodide  0.83
Potassium Nitrate  1900
Potassium Phosphate, Monobasic  170
Zinc Sulfate.7H2O  8.6
MES (Free Acid)  1000
myo-Inositol 100
Sucrose 30,000
Thiamine.HCl 0.4

Application Notes
Plant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuations during media preparation and culture.


References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.

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