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Introduction:
Contains the macro- and micronutrients as described by Lindemann et al. (1970); contains Sucrose and Vitamins.
L472 Lindemann Orchid Basal Medium
Properties
| Form: | Powder |
| Appearance: | White to Off-white Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 2.71 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Pr eparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro - and micronutrients as described by Lindemann et al. (1970); c ontains Sucrose and Vitamins. |
| Formula (mg/L) | |
|---|---|
| Aluminum Chloride.6H2O | 0.0561 |
| Ammonium Sulfate | 1000 |
| Boric Acid | 1.014 |
| Calcium Nitrate | 347.2 |
| Cupric Sulfate.5H2O | 0.019 |
| Ferric Citrate | 4.4 |
| Magnesium Sulfate, Anhydrous | 58.62 |
| Manganese Sulfate.H2O | 0.0515 |
| Nickel Chloride.6H2O | 0.0312 |
| Potassium Chloride | 1050 |
| Potassium Iodide | 0.099 |
| Potassium Phosphate, Monobasic | 135 |
| Zinc Sulfate.7H2O | 0.565 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Pyridoxine.HCl | 1 |
| Sucrose | 20,000 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Originally developed for the tissue culture of Cattleya orch
References
Lindemann EG, JE Gunckel, and OW Davidson. 1970. Meristem culture of Cattleya.
Amer.
Orchid Soc. Bull. 39:1002-1004