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Lindemann Orchid Basal Medium

  • Product ID: L472

Introduction:

Contains the macro- and micronutrients as described by Lindemann et al. (1970); contains Sucrose and Vitamins.

L472 Lindemann Orchid Basal Medium



Properties

Form: Powder
Appearance: White to Off-white Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 2.71 g/L
Storage Temp: 2-8° C
Storage Temp of Stock Solution: Pr eparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro - and micronutrients as described by Lindemann et al. (1970); c ontains Sucrose and Vitamins.
Formula (mg/L)
Aluminum Chloride.6H2O 0.0561
Ammonium Sulfate 1000
Boric Acid 1.014
Calcium Nitrate 347.2
Cupric Sulfate.5H2O 0.019
Ferric Citrate 4.4
Magnesium Sulfate, Anhydrous 58.62
Manganese Sulfate.H2O 0.0515
Nickel Chloride.6H2O 0.0312
Potassium Chloride 1050
Potassium Iodide 0.099
Potassium Phosphate, Monobasic 135
Zinc Sulfate.7H2O 0.565
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Pyridoxine.HCl 1
Sucrose 20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Originally developed for the tissue culture of Cattleya orch

References
Lindemann EG, JE Gunckel, and OW Davidson. 1970. Meristem culture of Cattleya. Amer. Orchid Soc. Bull. 39:1002-1004

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