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Linsmaier & Skoog Modified Basal Medium w/ Sucrose & Agar

  • Product ID: L467

Introduction:

Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1964). With 30 g/L sucrose & 7 g/L agar.

L467 Linsmaier & Skoog Basal Medium w/ 30 g/L Sucrose and 7 g/L Agar


Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO

Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 41.43 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965). pH = 4.25 – 5.25
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid  6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O  37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate  1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O  8.6
Agar 7000
myo-Inositol  100
Sucrose 30,000
Thiamine.HCl 0.4

Application Notes
Plant Tissue Culture Tested Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog.Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L.
Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.


References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.

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