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Introduction:
Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1964). With 30 g/L sucrose & 7 g/L agar.
L467 Linsmaier & Skoog Basal Medium w/ 30 g/L Sucrose and 7 g/L Agar
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 41.43 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965). pH = 4.25 – 5.25 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.26 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Agar | 7000 |
| myo-Inositol | 100 |
| Sucrose | 30,000 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog.Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L.
Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.