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Linsmaier & Skoog Modified Basal Medium w/ Sucrose & Agar , pH Adjusted & Buffered

  • Product ID: L452

Introduction:

Contains the macro-& micronutrients, & vitamins as described by L & S (1964). With 30 g/L sucrose & 7 g/L agar. pH adjusted and buffered.
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR's, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuation during media preparation and culture.

L452 Linsmaier & Skoog Modified Basal Medium w/ 30 g/L Sucrose & 7 g/L Agar pH Adjusted and Buffered



Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)


Properties

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 42.53 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965)
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous  332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O  0.025
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate  180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Hydroxide  100
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Agar 7000
MES (Free Acid)  1000
myo-Inositol 100
Sucrose 30,000
Thiamine.HCl 0.4

Application Notes
Plant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s or other supplements.
The addition of MES will help to stabilize pH fluctuations during media preparation and culture


References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.

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