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Lloyd & McCown Woody Plant Basal Medium with Vitamins

  • Product ID: L449

Introduction:

Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981).
This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium Nitrate has been eliminated from this medium and replaced with Potassium Sulfate.

L449 Lloyd & McCown Woody Plant Basal Medium



Synonym: WPM


Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 2.41g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate  400
Boric Acid 6.2
Calcium Chloride, Anhydrous  72.5
Calcium Nitrate 386
Cupric Sulfate.5H2O  0.25
Na2 EDTA.2H2O  37.3
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous  180.7
Manganese Sulfate.H2O  22.3
Molybdic Acid (Sodium Salt) .2H2O  0.25
Potassium Phosphate, Monobasic  170
Potassium Sulfate  990
Zinc Sulfate.7H2O 8.6
Glycine (Free Base)  2
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine.HCl 0.5
Thiamine.HCl  1

Application Notes
Plant Tissue Culture Tested
Plant species: Kalmia latifolia (Mountain Laurel), Rhododendron spp. This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium nitrate has been eliminated from this medium and replaced with potassium sulfate. The levels of ammonium nitrate are ¼x compared to MS. Nitrogen is also provided by calcium nitrate.


References
Lloyd, G and BH McCown. 1981. Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc. 30:421-427.

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