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Introduction:
Contains the Woody plant medium macro- and micronutrients as described by Lloyd & McCown (1981).
This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium Nitrate has been eliminated from this medium and replaced with Potassium Sulfate.
L154 Lloyd & McCown Woody Plant Basal Salt Mixture
Synonym: WPM Basal Salts
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 2.30g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 400 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 72.5 |
| Calcium Nitrate | 386 |
| Cupric Sulfate.5H2O | 0.25 |
| Na2 EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 22.3 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Phosphate, Monobasic | 170 |
| Potassium Sulfate | 990 |
| Zinc Sulfate.7H2O | 8.6 |