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King's B Medium

  • Product ID: K5013

Introduction:

Non-selective general bacterial subculturing medium.
Often used for the detection & culture of pseudomonads such as Pseudomonas aeruginosa and Psuedomonas syringae by the addition of cephalexin & nystatin (see product info sheet for details) and for distinguishing fluorescent pseudomonads from nonfluorescent pseudomonads.
10 mL/L of Glycerol (G381) should be added to the medium before autoclaving.


K5013 King’s B Medium


Synonyms: KB Medium, KB Agar, King’s Agar B, King’s Base Medium; KBM; KB; King's B Agar; Pseudomonas Agar F


Properties

Form: Powder
Appearance: Off-White to Tan
Application: Phytopathology, Microbiology, Seed Testing, Plant Defense & Immunity
Solubility: Partially Soluble in Cold Water, Soluble in Boiling Water
Typical Working Concentration: 37.23 g/L
Storage Temp: Room Temperature
Other Notes: Add 10-15 mL/L of Glycerol (Prod # G381) prior to autoclaving for carbon source.
Add 50 mg Cephalexin (Prod # C2112 or C1970) and 35 mg Nystatin (Prod # N581) per liter to select for Pseudomonads (Vogel & Bonner, 1956)
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